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A scientific vacation and superresolution adventure at the Janelia Farm Advanced Imaging Center

posted Sep 10, 2015, 3:51 PM by Rod O'Connor
As France was settling into its summer vacation, PhD student Lynn Carr, Philippe Leveque and Rod O'Connor packed up pulse generators, RF connectors, cells and reagents and jumped on a plane to visit the Advanced Imaging Center of the Janelia Farm Research Campus of the Howard Hughes Medical Institute in Ashburn, Virginia. Two intense weeks of exciting experiments followed at the AIC, which was established in recent years to allow visiting scientists to conduct experiments using the unique instruments developed at Janelia, including imaging systems capable of Interferometric photoactivated localization microscopy (iPALM), Lattice light sheet microscopy,  Single-molecule total internal reflection fluorescence (sm-TIRF) microscopy, Aberration-corrected multifocus microscopy (acMFM), and Live cell multicolor structured illumination microscopy (SIM). We had the pleasure of using the multicolor 3D-SIM scope in standard and TIRF mode, which showed us some of the crispest and most beautiful images of mitochondria and cytoskeleton that we have ever seen. The AIC visiting program was simply fantastic with great staff, support and logistics. We were proud to have been the first team from France to participate in this new program and have a few Terabytes of data to now crunch through. Many thanks to HHMI and the Gordon and Betty Moore Foundation for setting up this great program.